The efficiency of keratin extraction from human hair by sulfitolysis

Biotechnology, Ecology & Sustainable Development


First and Last Name Academic degree E-mail Affiliation
Vasylyna Mykhaliuk Ph.D. vasylyna.v.m [at] Institute of Animal Biology of NAAS
Lviv, Ukraine
Viktoriya Havryliak Sc.D. havvita [at] Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology, Institute of Chemistry and Chemical Technology, Lviv Polytechnic National University
Lviv, Ukraine

I and my co-authors (if any) authorize the use of the Paper in accordance with the Creative Commons CC BY license

First published on this website: 02.09.2019 - 23:50

This study has a twofold objective: first, to determine the efficiency of keratin extraction from human hair, by following the method suggested by Isarankura Na Ayutthaya et al. (2015); and second, to establish the electrophoretic profile of the obtained proteins. It was investigated that the efficiency of keratin extraction was 50.24 ± 8.21%. The electrophoretic analysis of keratins has shown two protein fractions in the range of 40-60 kDa, which belongs to the proteins of the intermediate filaments.


1. Naito S., Arai K. (1996). Type and location of SS linkages in human hair and their relation to fiber properties in water. Journ. App. Polym. Sci., 61:1918-2113.

2. Yamauchi K., Yamauchi A., Kusunoki T., Kohda A., Konishi Y. (1996). Preparation of stable aqueous solution of keratins, and physiochemical and biodegradational properties of films.J. Biomed. Mater. Res.,31:439–444.

3. Zoccola M., Aluigi A., Vineis C., Tonin C., Ferrero F., Piacentino M.G. (2008). Study on cast membranes and electrospun nanofibers made from keratin/fibroin blends. Biomacromolecules., 9:2819–2825.

4. Tachibana A., Kaneko S., Tanabe T., Yamauchi K. (2005). Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation. Biomaterials., 26:297–302.

5. Sierpinski P., Garrett J., Ma J., Apel P., Klorig D., Smith T., Koman L.A., Atala A., Van Dyke M. (2008). The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials., 29:118–128.

6. Isarankura Na Ayutthaya, Tanpichai S., Wootthikanokkhan J. (2015) Keratin Extracted from Chicken Feather Waste: Extraction, Preparation, and Structural Characterization of the Keratin and Keratin/Biopolymer Films and Electrospuns. Journal o f Polymers., 23(4).

Full text


Ivan Tymchuk
reviewer, secretary

Dear Authors,


Your paper has been accepted!

We are glad to inform you that your conference paper has been accepted to participate in the 9th International Youth Science Forum «Litteris et Artibus» (November 21-23, 2019, Lviv, Ukraine)! Your paper will be published in digital proceedings with ISSN and DOI on the website

Please, answer the following participation questions:

•   Are you going to take part in the conference in person?

•   Do you require official invitation letter from Lviv Polytechnic? (The invitation letter will be scanned and sent to your e-mail).

Presentation delivery regulations:

Conference participants will present their work on posters. Content and design of posters is arbitrary, but it should include the basic provisions of your paper: author names, title, main points, equations, figures etc. as well as exclude solid color, gradient or other costly to print backgrounds. The poster's upper part may include some information about your organization (title and logo, brief contact information).

Selected papers will be presented orally.

We also ask you to cover the conference fee till October 25, 2019:

  • International participants – 100 EUR;
  • Participants from Ukraine – 450 UAH.

Conference fees include online paper publication and organizational expenses. Cost of accommodation, meals and social program are not included. 

Please note, that invitation will be sent and proceedings will be implemented only after 100% payment fee.

If you need help with accommodation, please send a request to the Secretary. Invitations to lunch will be available for purchase at the registration desk.

The conference fee’s payment details:

Account details for payment of the participation fee from Ukraine participants (in UAH):

Одержувач: Первинна профспілкова організація студентів та аспірантів Національного університету «Львівська політехніка»

Банк: Акціонерний банк «Південний»

Код установи банку: 20953647

МФО: 328209

код за ЄДРПОУ (ІПН): 20846213

Номер рахунку: 26004010050168

Призначення платежу: П.І.Б, оргвнесок за участь у конференції «LEA-2019»


Account details for payment of the participation fee by international participants (in EURO):

Beneficiary: Lviv Polytechnic Alumni Association

IBAN: UA753805820000026002010325122




Corraccount No.: 400 8866 75800

With Correspondent Bank: COMMERZBANK AG, Frankfurt am Main, Germany



The full Forum’s program will be published on the before November 1, 2019.

Looking forward to receiving your poster! See you in Lviv soon!


Best regards,

Ivan Tymchuk,

Secretary BESD

Tue, 10/22/2019 - 09:48