The practical significance of the work is to search new ways and methods of preservation ancient trees of Ukraine. Due to the natural aging condition this centuries-old trees are characterized by high risk of extinction as a result of biotic, abiotic and anthropogenic factors. Using of biotechnological methods, such as micropropagation can solve the problem of maintaining the unique gene pool of woody plants, study their ecosystem role and development of ornamental horticulture. In this work was shown peculiarities of getting aseptic culture and first stages of micropropagation of ancient trees
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