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Proliferation of Rindera graeca transgenic roots in oscillatory rocked disposable bioreactor

Optimization of biochemical processes and bioengineering
3rd International Scientific Conference «Chemical Technology and Engineering»: Proceedings – June 21–24th, 2021, Lviv, Ukraine – Lviv: Lviv Polytechnic National University, 2021, pp. 164–165

DOI: https://doi.org/10.23939/cte2021.01.164

Authors

First and Last Name Academic degree E-mail Affiliation
Kamil Wierzchowski No kamil.wierzchowski.dokt [at] pw.edu.pl Faculty of Chemical and Process Engineering, Warsaw University of Technology
Warsaw, Poland
Mateusz Kawka No mateusz.kawka [at] wum.edu.pl Faculty of Pharmacy, Medical University of Warsaw
Warsaw, Poland
Katarzyna Sykłowska-Baranek Ph.D. katarzyna.syklowska-baranek [at] wum.edu.pl Faculty of Pharmacy, Medical University of Warsaw
Warsaw, Poland
Maciej Pilarek Ph.D. maciej.pilarek [at] pw.edu.pl Faculty of Chemical and Process Engineering, Warsaw University of Technology
Warsaw, Poland

I and my co-authors (if any) authorize the use of the Paper in accordance with the Creative Commons CC BY license

First published on this website: 29.04.2021 - 16:26
Abstract

Plant biomass is a commonly known source of pharmaceutically valuable compounds. The study aimed to investigate the influence of wave-type agitation conditions on the proliferation of Ringera graeca hairy roots in the disposable bioreactor. Transgenic roots morphology, fresh biomass increases, and yield of extracellularly secreted bioactive compounds have been recognized.

Keywords
disposable (single-use) bioreactorwave-assisted agitationtransgenic (hairy) rootsaerationbioprocess intensification
References
  1. Ochoa-Villarreal, M., Howat, S., Hong, S., Jang, M. O., Jin, Y.-W., Lee, E.-K., Loake, G. L. (2016). Plant cell cultures strategies for the production of natural products. BMB Reports, 49(3), 149-158. https://doi.org/10.5483/BMBRep.2016.49.3.264
  2. Nowak, B., Kawka, M., Wierzchowski, K., Sykłowska-Baranek, K., Pilarek, M. (2021). MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones. Journal of Functional Biomaterials, 12, 19. https://doi.org/10.3390/jfb12010019
  3. Wierzchowski, K., Grabowska, I., Pilarek, M., (2020). Efficient propagation of suspended HL‑60 cells in a disposable bioreactor supporting wave‑induced agitation at various Reynolds number. Bioprocess and Biosystems Engineering, 43, 1973–1985. https://doi.org/10.1007/s00449-020-02386-6.
First published Full Text (29.04.2021 - 16:26)
Official paper